Substrate-dependent modulation of 3D spheroid morphology self-assembled in mesenchymal stem cell-endothelial progenitor cell coculture

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• 作者：Shan-hui Hsu^a ; ^b ; ^{shhsu@ntu.edu.tw" class="auth_mail} ; ^{shhsu@mail.nchu.edu.tw" class="auth_mail} ; Tung-Tso Ho^a ; Nien-Chi Huang^a ; Chao-Ling Yao^c ; Luen-Hau Peng^c ; Niann-Tzyy Dai^d
• 关键词：Mesenchymal stem cells (MSCs) ; Coculture ; Endothelial progenitor cells (EPCs) ; Cell&ndash ; cell interaction ; Integrin ; Notch signaling
• 刊名：Biomaterials
• 出版年：August 2014
• 年：2014
• 卷：35
• 期：26
• 页码：7295-7307
• 全文大小：5352 K

文摘

The structural evolution of three-dimensional spheroids self-assembled from two different types of cells on selective biomaterials is demonstrated in this study. The two types of cells involved in the self-assembly are human mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs). When seeded in different population ratios, they can create a variety of cellular patterns on different biomaterial substrates. When the two populations are matched in initial numbers, they are self-assembled in co-spheroids with different morphologies (i.e. randomly mixed, bumped, or concentric spheroids). The morphologies are influenced by the specific cell-substrate interaction possibly through integrin signaling, as well as a substrate-dependent regulation of heterophilic cell–cell interaction possibly through Notch signaling. In particular, the self-assembled core–shell concentric spheroids from adipose-derived MSCs and EPCs show a greater angiogenic effect in vitro. This study reveals the possibility to modulate the self-assembled morphology as well as the effect of cocultured cells by changing the cell culture substratum.