Trypanosoma cruzi contains two galactokinases; molecular and biochemical characterization
详细信息 查看全文
- 作者：Á ; ngel E. Lobo-Rojasa ; Eglys B. Gonzá ; lez-Marcanoa ; Edward A. Valera-Veraa ; Hé ; ctor R. Acostaa ; Wilfredo A. Quiñ ; onesa ; Richard J.S. Burchmoreb ; Juan L. Concepció ; na ; Ana J. Cá ; ceresa ; acaceres@ula.ve" class="auth_mail" title="E-mail the corresponding author
- 关键词：TcGALKs ; (TcGALK-1 ; TcGALK-2) galactokinases from T. cruzi ; HK ; hexokinase ; GEF ; glycosome-enriched fraction ; IMAC ; immobilized metal affinity chromatography ; PTS-1 ; peroxisome-targeting signal type 1 ; ORF ; open-reading frame
- 刊名：Parasitology International
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：65
- 期：5
- 页码：472-482
- 全文大小：923 K
文摘
- •
Two different Trypanosoma cruzi galactokinase genes were cloned and expressed in Escherichia coli.
- •
Both galactokinase genes are expressed in Trypanosoma cruzi epimastigotes.
- •
Both galactokinase enzymes are present inside glycosomes.
- •
20">Trypanosoma cruzi may have an alternative pathway for UDP-galactose synthesis.
- •
25">Epimastigotes can grow in a medium supplemented with galactose as carbon source.