Challenges and opportunities of using liquid chromatography and mass spectrometry methods to develop complex vaccine antigens as pharmaceutical dosage forms
详细信息 查看全文
- 作者：John M. Hickey ; Neha Sahni ; Ronald T. Toth IV ; Ozan S. Kumru ; Sangeeta B. Joshi ; C. Russell Middaugh ; David B. Volkin ; volkin@ku.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：LC ; liquid&ndash ; chromatography ; MS ; mass spectrometry ; HA ; hemagglutinin ; NA ; neuraminidase ; SRID ; single radial immunodiffusion assay ; WHO ; World Health Organization ; RP ; reversed-phase chromatography ; SEC ; size-exclusion chromatography ; IEX ; ion-exchange chromatography ; RSD ; relative standard deviation ; VLP ; virus-like particle ; TEM ; transmission electron microscopy ; HPV ; human papillomavirus ; HBV ; hepatitis B virus ; HBsAg ; hepatitis B surface antigen ; HEV ; hepatitis E virus ; MW ; molecular we
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：1 October 2016
- 年：2016
- 卷：1032
- 期：Complete
- 页码：23-38
- 全文大小：3597 K
文摘
- m" id="list_lis0005">
- •
High-resolution analytical methods are critical to develop complex vaccine antigens.
- •
Liquid chromatographic and mass spectrometric methods are reviewed.
- •
Some of the advantages and challenges of using these methods are also discussed.