The reaction with immobilized HRP was faster than with SBP at all the enzyme concentrations assayed. However, at enzyme concentrations lower than 0.028 mg/ml, 7 % and 20 % more phenol was removed with SBP than with HRP. This behaviour changed as the enzyme dose increased. Thus, the addition of 0.039 mg/ml of either immobilized SBP or HRP eliminated 77 % of the initial phenol. Increasing the amount of hydrogen peroxide added to the reactor led to higher conversions and more than 95 % of the initial phenol present in the solution was eliminated with immobilized SBP at an H2O2/phenol molar ratio of 1.5 for all the phenol concentrations studied. However, immobilized HRP was more susceptible to inactivation/inhibition effects and its phenol removal capacity was lower (less than 93 % ).