- 作者：Shinsuke Matsumoto ; Hisashi Iwata ; Koyo Shirahashi ; Masanao Saio ; Yukio Umeda ; Tsutomu Marui ; Narihiro Ishida ; Masaki Kimura ; Takuya Sugimoto ; Hideaki Manabe ; Hirofumi Takemura
- 关键词：extensive pulmonary resection ; right ventricular hypertrophy ; right heart failure ; G-CSF
- 刊名：Journal of Surgical Research
- 出版年：2010
- 出版时间：August 2010
- 年：2010
- 卷：162
- 期：2
- 页码：153-159
- 全文大小：638 K
Background
The objective of the present study was to investigate the effects of granulocyte colony-stimulating factor (G-CSF) on right ventricular hypertrophy following extensive pulmonary resection in rats.Materials and Methods
Adult rats were divided into four groups: (1) Group S (right thoracotomy only); (2) Group L (right three lobectomy); (3) Group LG10 (Group L + G-CSF [10 μg/kg/d]); and (4) Group LG100 (Group L + G-CSF [100 μg/kg/d]). At postoperative day 21, weight ratio of the right ventricular to the left ventricle plus septum (RV/LV + S, indicator of right ventricular hypertrophy) were measured, and a histopathological study was conducted to determine percentage wall thickness of peripheral pulmonary arteries and proliferating cell nuclear antigen labeling index (indicator of oxidative DNA damage) of right ventricles.
Results
Mean RV/LV + S for Group S was 0.27 ± 0.02, significantly smaller than that for the lobectomy groups (Group L, LG10, LG100; 0.47 ± 0.05, 0.35 ± 0.02, 0.38 ± 0.05). G-CSF significantly suppressed right ventricular hypertrophy. Mean medial wall thickness of peripheral pulmonary arteries for Group S was 13.6 % ± 4.9 % , significantly smaller than that for Group L (22.9 % ± 9.6 % ). Compared with Group L, G-CSF reduced medial wall thickness (LG10, 17.6 % ± 9.5 % ; LG100, 18.0 % ± 11.2 % ). Incidence of proliferating cell nuclear antigen positive nuclei for Group S was 1.07 % ± 0.49 % , significantly smaller than that for Group L (13.77 % ± 5.87 % ). G-CSF significantly reduced the incidence of proliferating cell nuclear antigen positive nuclei (LG10, 4.04 % ± 2.14 % ; LG100, 3.18 % ± 1.66 % ).
Conclusions
G-CSF administration not only reduce medial wall thickness of peripheral pulmonary arteries but also directly protect cardiomyocytes of the right ventricle, thus suppressing right ventricular hypertrophy. These results suggest that low-dose G-CSF administration can prevent right heart failure following extensive pulmonary resection.