In vivo

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• 作者：Hidetoshi Akimoto ; Hyuck Joon Kwon ; Michitaka Ozaki ; Kazunori Yasuda ; Ken-ichi Honma ; Yoshihiro Ohmiya
• 关键词：Bone marrow-derived cells ; Brain inflammation ; In vivo bioluminescence imaging
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2009
• 出版时间：20 March 2009
• 年：2009
• 卷：380
• 期：4
• 页码：844-849
• 全文大小：2334 K

文摘

It has been accepted that bone marrow cells infiltrate the brain and play important roles in neuroinflammation. However, there is no good tool for the visualization of these cells in living animals. In this study, we generated mice that were transplanted with GFP- or luciferase-expressing bone marrow cells, and performed in vivo fluorescence imaging (FLI) and in vivo bioluminescence imaging (BLI) to visualize the infiltrated cells. Brain inflammation was induced by intrahippocampal injection of lipopolysaccharide (LPS). Immunohistochemical investigation demonstrated an increase in the infiltration of bone marrow cells into the hippocampus because of the LPS injection and differentiation of the infiltrated cells into microglia, but not into neurons or astrocytes. BLI, but not FLI, successfully detected an increase in signal intensity with the LPS injection, and the increase of BLI coincided with that of luciferase activity in hippocampus. BLI could quantitatively and continuously monitor bone marrow-derived cells in vivo.