Increase in hepatic mRNA expression of coagulant factors in type 2 diabetic model mice

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• 作者：Murakami ; Hidetomo ; Okazaki ; Masako ; Amagasa ; Hidetoshi ; Oguchi ; Katsuji
• 关键词：Fibrinogen ; Prothrombin ; Diabetes ; mRNA ; RT-PCR
• 刊名：Thrombosis Research
• 出版年：2003
• 出版时间：2003
• 年：2003
• 卷：111
• 期：1-2
• 页码：81-87
• 全文大小：444 K

文摘

Aim: The purpose of the study is to investigate whether hypercoagulation in diabetes is observed not only in increased plasma levels of the coagulative factors but also in increased hepatic mRNA levels. Materials and methods: The age-related changes of coagulation factors were determined using KK and KK-A^y mice as a model for human type 2 diabetes mellitus. Expression of the α-, β- and γ-chains of fibrinogen-mRNA and prothrombin-mRNA from the mouse liver was examined by reverse transcription–polymerase chain reaction (RT-PCR). Results: Hemoglobin A_1c (HbA_1C), plasma fibrinogen, triglyceride and insulin levels increased apparently, especially in KK-A^y mouse at 4 months old. The mRNA levels of γ-chain of fibrinogen significantly increased at 3 and 4 months of age in both mice. The mRNA levels of α- and β-chains of fibrinogen and prothrombin were significantly increased in 4-month-old KK-A^y mouse. Conclusions: These results suggest that the increase in hepatic mRNA expression of coagulant factors contributes to the hypercoagulable state in type 2 diabetic mice.