Sensitivity improvement of a sandwich-type ELISA immunosensor for the detection of different prostate-specific antigen isoforms in human serum using electrochemical impedance spectroscopy and an ordered and hierarchically organized interfacial supramolecular architecture
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- 作者：Gabriela Guadalupe Gutié ; rrez-Zú ; ñ ; iga ; José ; Luis Herná ; ndez-Ló ; pez ; jhernandez@cideteq.mx" class="auth_mail" title="E-mail the corresponding author
- 关键词：Electrochemical impedance spectroscopy ; Enzyme-linked immunosorbent assay ; Immunosensor ; Interfacial properties ; Non-specific protein adsorption ; Prostate cancer
- 刊名：Analytica Chimica Acta
- 出版年：2016
- 出版时间：1 January 2016
- 年：2016
- 卷：902
- 期：Complete
- 页码：97-106
- 全文大小：2150 K
文摘
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The detection of different PSA isoforms using a capacitance affinity biosensor is reported.
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Non-specific protein adsorption could be minimized and assays against t– and f–PSA could be carried out in 100% human sera.
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The electrochemical format is simple, ergonomic, rapid, highly sensitive and selective.
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The interfacial supramolecular architecture provides a promising platform for forensic applications.