GSK3尾 negatively regulates HIF1伪 mRNA stability via nucleolin in the MG63 osteosarcoma cell line

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• 作者：Dong-dong Cheng ; Hai-guang Zhao ; Yun-song Yang ; Tu Hu ; Qing-cheng Yang
• 关键词：HIF1伪 ; hypoxia-inducible factor 1伪 ; GSK3尾 ; glycogen synthase kinase 3 beta ; VEGF ; vascular endothelial growth factor ; EPO ; erythropoietin ; GLUT1 ; glucose transporter 1 ; ARNT ; aryl hydrocarbon receptor nuclear translocator ; ODD ; oxygen-dependent degradation ; VHL ; von Hippel-Lindau ; SDS&ndash ; PAGE ; sodium dodecyl sulfate polyacrylamide gel electrophoresis ; Chx ; cycloheximide ; siRNA ; small interfering RNA
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：10 January, 2014
• 年：2014
• 卷：443
• 期：2
• 页码：598-603
• 全文大小：1260 K

文摘

Hypoxia-inducible factor 1伪 (HIF1伪) is a transcription factor involved in the growth, invasion and metastasis of malignant tumors. Glycogen synthase kinase 3 beta (GSK3尾) is a protein kinase involved in a variety of signaling pathways, such as the Wnt and NF-魏B pathways; this kinase can affect tumor progress through the regulation of transcription factor expression and apoptosis. Recent studies showed that GSK3尾 was involved in the expression of HIF1伪. However, the effect of GSK3尾 on HIF1伪 expression in osteosarcoma cells remains unknown. To understand the relationship between GSK3尾 and HIF1伪 comprehensively, small RNA interference techniques, Western blot analyses, quantitative real-time PCR analyses and luciferase assays were used in our study. Experimental data revealed that inhibition of GSK3尾 could increase HIF1伪 protein levels and expression of its target genes by increasing the stability of the HIF1伪 mRNA, not by affecting the HIF1伪 protein stability, and that this process could be mediated by nucleolin.