Detection of Neorickettsia risticii from various freshwater snail species collected from a district irrigation canal in Nevada County, California

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• 作者：Nicola Pusterla ; Daniel Hagerty ; Samantha Mapes ; Josh VanGeem ; Lindsey T. Groves ; Mario Dinucci ; Langdon C. Fielding ; Jill C. Higgins
• 关键词：Neorickettsia risticii ; Equine neorickettsiosis ; Horse ; Freshwater snails ; Irrigation canal ; Potomac horse fever
• 刊名：The Veterinary Journal
• 出版年：2013
• 出版时间：August, 2013
• 年：2013
• 卷：197
• 期：2
• 页码：489-491
• 全文大小：1065 K

文摘

This study investigated the role of a district irrigation canal in Nevada County, California, USA, as the point source of infection for Neorickettsia risticii, causative agent of equine neorickettsiosis (EN). A total of 568 freshwater snails comprising Juga spp., Planorbella subcrenata (Carpenter, 1857) (Rough Rams-horn), Physella virgata (Gould, 1855) (Protean Physa) and feces from three horses with EN were collected and tested for N. risticii by real-time PCR. A total of four freshwater snails tested PCR positive for N. risticii. Phylogenetic analysis showed 99.8-100 % homology between the different snail and horse N. risticii isolates. This study represents the first report of infection with N. risticii in Planorbella subcrenata and suggests that the irrigation canal was the aquatic environment responsible for the spread of N. risticii.