PyNPase expression in human colon cancer

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• 作者：Haba ; Akinao ; Monden ; Takushi ; Sekimoto ; Mitsugu ; Ikeda ; Kimimasa ; Izawa ; Hikaru ; Kanou ; Toshiyuki ; et. al.
• 关键词：PyNPase ; Colorectal cancer ; Stroma ; Protein extraction ; Western blot analysis
• 刊名：Cancer Letters
• 出版年：1998
• 出版时间：January 9, 1998
• 年：1998
• 卷：122
• 期：1-2
• 页码：85-92
• 全文大小：704 K

文摘

Although colorectal cancer tissue is rich in pyrimidine nucleoside phosphorylase (PyNPase), there is no consensus as to whether cancer cells or stromal cells predominately express PyNPase. We micro-dissected OCT compound embedded frozen tissue sections into epithelial and stromal components and then analyzed the extracted samples separately. The PyNPase expression level was higher in stromal cells than in cancer cells and the difference increased with inflammation induced by the immunostimulator OK432. These results suggest that stromal cells are the major PyNPase source in colorectal cancer.