Utilization of recombinant Trichoderma reesei expressing Aspergillus aculeatus 尾-glucosidase I (JN11) for a more economical production of ethanol from lignocellulosic biomass

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• 作者：Treesukon Treebupachatsakul¹ ; Koki Shioya¹ ; Hikaru Nakazawa¹ ; Takashi Kawaguchi² ; Yasushi Morikawa¹ ; Yosuke Shida¹ ; Wataru Ogasawara¹ ; ^{owataru@vos.nagaokaut.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Hirofumi Okada¹ ; ^&dagger ;
• 关键词：Trichoderma reesei ; Aspergillus aculeatus BGL I ; Simultaneous saccharification and fermentation ; Separate hydrolysis and fermentation ; Ethanol
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：120
• 期：6
• 页码：657-665
• 全文大小：560 K

文摘

The capacity of Trichoderma reesei cellulase to degrade lignocellulosic biomass has been enhanced by the construction of a recombinant T. reesei strain expressing Aspergillus aculeatus 尾-glucosidase I. We have confirmed highly efficient ethanol production from converge-milled Japanese cedar by recombinant T. reesei expressing A. aculeatus 尾-glucosidase I (JN11). We investigated the ethanol productivity of JN11 and compared it with the cocktail enzyme T. reesei PC-3-7 with reinforced cellobiase activity by the commercial Novozyme 188. Results showed that the ethanol production efficiency under enzymatic hydrolysis of JN11 was comparable to the cocktail enzyme both on simultaneous saccharification and fermentation (SSF) or separate hydrolysis and fermentation (SHF) processes. Moreover, the cocktail enzyme required more protein loading for attaining similar levels of ethanol conversion as JN11. We propose that JN11 is an intrinsically economical enzyme that can eliminate the supplementation of BGL for PC-3-7, thereby reducing the cost of industrial ethanol production from lignocellulosic biomass.