A G-quadruplex-binding macrodomain within the 鈥淪ARS-unique domain鈥?is essential for the activity of the SARS-coronavirus replication-transcription complex
详细信息 查看全文
- 作者：Yuri Kusova ; b ; koussov@biochem.uni-luebeck.de" class="auth_mail" title="E-mail the corresponding author ; Jinzhi Tana ; tanjinzhi@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Enrique Alvarez1 ; c ; ealvarezmba@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Luis Enjuanesc ; L.enjuanes@cnb.csic.es" class="auth_mail" title="E-mail the corresponding author ; Rolf Hilgenfelda ; b ; hilgenfeld@biochem.uni-luebeck.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：SARS-CoV replicon ; SARS-unique domain ; Macrodomain ; X-domain ; Reverse genetics ; G-quadruplex ; MERS-CoV
- 刊名：Virology
- 出版年：2015
- 出版时间：October 2015
- 年：2015
- 卷：484
- 期：Complete
- 页码：313-322
- 全文大小：1620 K
文摘
- •
A SARS-CoV replicon encoding Renilla luciferase as reporter protein is constructed.
- •
The role of three macrodomains for the replication/transcription complex is analyzed.
- •
In contrast to macrodomains X and SUD-N, SUD-M is found indispensable for replication.
- •
Site-directed mutagenesis identifies charged SUD-M residues required for replication.
- •
These residues have previously been shown to be involved in G-quadruplex binding.