A novel approach to automated genotyping of Mycobacterium tuberculosis using a panel of 15 MIRU VNTRs
详细信息 查看全文
- 作者：Tomoshige Matsumoto ; tom_matsumoto@sutv.zaq.ne.jp ; tomoshige.matsumoto@gmail.com ; Yuriko Koshii ; Kazu Sakane ; Tomomi Murakawa ; Yukio Hirayama ; Hisako Yoshida ; Masashi Kurokawa ; Yoshitaka Tamura ; Takayuki Nagai ; Ichiro Kawase
- 关键词：MIRU ; mycobacterial interspersed repetitive units ; VNTR ; variable number of tandem repeats ; RFLP ; restriction fragment length polymorphism
- 刊名：Journal of Microbiological Methods
- 出版年：2013
- 出版时间：June, 2013
- 年：2013
- 卷：93
- 期：3
- 页码：239-241
- 全文大小：457 K
文摘
In this study, we present a new approach to variable number tandem repeats (VNTR) analysis using the QIAxcel capillary electrophoresis system and a software-integrated peak calling function. Allelic ladders representing 15 mycobacterial interspersed repetitive units (MIRU)-VNTR loci were used to define peak calling tables thereby enabling high precision Mycobacterium tuberculosis strain identification.