Smart conferring of nuclease resistance to DNA by 3′-end protection using 2′,4′-bridged nucleoside-5′-triphosphates

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• 作者：Masayasu Kuwahara ; Satoshi Obika ; Hidetoshi Takeshima ; Yoshihiko Hagiwara ; Jun-ichi Nagashima ; Hiroaki Ozaki ; Hiroaki Sawai ; Takeshi Imanishi
• 关键词：2′ ; 4′ ; -Bridged nucleoside-5′ ; -triphospate ; Bridged nucleic acid (BNA) ; Terminal deoxynucleotidyl transferase (TdT) ; Nuclease resistance
• 刊名：Bioorganic and Medicinal Chemistry Letters
• 出版年：2009
• 出版时间：1 June 2009
• 年：2009
• 卷：19
• 期：11
• 页码：2941-2943
• 全文大小：249 K

文摘

Incorporation of 2′,4′-bridged nucleotides into the 3′-end of oligodeoxyribonucleotide (ODN) was examined using terminal deoxynucleotidyl transferase (TdT). The three types of 2′,4′-bridged nucleoside-5′-triphospates with different bridging structures used were incorporated efficiently into the 3′-end of DNA by TdT, although only single nucleotide incorporation was observed. Nuclease resistance was conferred on DNA, depending on the types of bridging nucleotides added.