Effect of 3′-end capping of aptamer with various 2′,4′-bridged nucleotides: Enzymatic post-modification toward a practical use of polyclonal aptamers

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• 作者：Yuuya Kasahara ; Shunsuke Kitadume ; Kunihiko Morihiro ; Masayasu Kuwahara ; Hiroaki Ozaki ; Hiroaki Sawai ; Takeshi Imanishi ; Satoshi Obika
• 关键词：2′ ; 4′ ; -Bridged nucleoside-5′ ; -triphosphate ; Bridged nucleic acid (BNA) ; Nuclease resistance ; Post-modification ; Terminal deoxynucleotidyl transferase (TdT)
• 刊名：Bioorganic and Medicinal Chemistry Letters
• 出版年：2010
• 出版时间：1 March 2010
• 年：2010
• 卷：20
• 期：5
• 页码：1626-1629
• 全文大小：569 K

文摘

The capping of the 3′-ends of thrombin binding aptamers (TBAs) with bridged nucleotides increased the nuclease resistances and the stabilities in human serum. The binding abilities of the aptamers were not affected by the capping. The capping could be simply executed via a one step enzymatic process using 2′,4′-bridged nucleoside 5′-triphosphate and terminal deoxynucleotidyl transferase.