Analysis of enterovirus genotypes using single-strand conformation polymorphisms of polymerase chain reaction products
详细信息 查看全文
- 作者：Fujioka ; Shigekazu ; Koide ; Hisashi ; Kitaura ; Yasushi ; Duguchi ; Hirofumi ; Kawamura ; Keishiro
- 关键词：Enterovirus ; Genotyping ; Polymerase chain reaction ; Single-strand conformation polymorphism analysis
- 刊名：Journal of Virological Methods
- 出版年：1995
- 出版时间：February, 1995
- 年：1995
- 卷：51
- 期：2-3
- 页码：253-258
- 全文大小：327 K
文摘
Enterovirus genotypes were identified rapidly by reverse transcription-polymerase chain reaction (RT-PCR) followed by single-strand conformation polymorphism (SSCP) analysis. The primer pair was chosen from the highly conserved sequence at the 5′ non-coding region of enterovirus genomes. RT-PCR amplified a 154 bp sequence in all samples from 14 serotypes of enteroviruses, including group A and B Coxsackie viruses, echoviruses and polioviruses. SSCP analysis of these products revealed different electrophoretic profiles. Thus, SSCP analysis will be useful for differentiating the genotypes of enteroviruses, and may be applicable for rapid diagnosis of enteroviral infection.