HlCLec with triple CRDs was identified from Haemaphysalis longicornis.
HlCLec mRNA is upregulated during blood feeding and highest in the midgut and ovary.
Each CRD showed binding ability to both Escherichia coli and S. aureus.
Each CRD had no growth inhibitory effeect to both E. coli and S. aureus.
E. coli injection after HlCLec silencing caused significant decrease in tick survival.