Enhancer activity-based identification of functional enhancers using zebrafish embryos
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- 作者：Tomohito Taminato1 ; Daisuke Yokota1 ; Soh Araki ; Hiroki Ovara ; Kyo Yamasu ; Akinori Kawamura ; akawamur@mail.saitama-u.ac.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：Enhancer ; Cis-regulatory element ; Tailbud ; Zebrafish
- 刊名：Genomics
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：108
- 期：2
- 页码：102-107
- 全文大小：1167 K
文摘
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We developed an enhancer activity-based screening approach, ChIP-Injection.
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ChIP-injection identifies the enhancers by injection of the H3K27Ac-associated DNA with the EGFP reporter into zebrafish embryos.
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ChIP-Injection enables simple and efficient identification and characterization of functional enhancers.