Specific regulation of nucleocytoplasmic distribution of poly(C)-binding protein gene mRNA in mouse development

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• 作者：Hidai ; Chiaki ; Ohno ; Masako ; Kazama ; Hiromi ; Ikeda ; Hiromi ; Kasanuki ; Hiroshi ; Kokubun ; Shinichiro ; et. al.
• 关键词：α ; CP2 ; mRNA distribution ; Nucleus ; Cytoplasm ; Endothelial cell ; Cardiac myocyte ; Development
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2003
• 出版时间：September 19, 2003
• 年：2003
• 卷：309
• 期：2
• 页码：339-343
• 全文大小：176 K

文摘

Post-transcriptional regulation plays a pivotal role in gene expression. In this study, the intracellular distribution of the murine cytoplasmic poly(C)-binding protein (αCP2) gene transcript was investigated. The nucleocytoplasmic mRNA distribution of αCP2 was shown to change throughout the course of mouse development. Furthermore, in situ hybridization of the embryo revealed that the αCP2 transcript was widely distributed in the cytoplasm of endothelial cells and cardiac myocytes, but accumulated in the nuclei of other cells. In the adult, αCP2 was ubiquitously expressed and αCP2 mRNA was found to accumulate in the nucleus. In vitro experiments showed that the nucleocytoplasmic mRNA distribution of αCP2 mRNA was distinct from that of the GAPDH gene used as an internal control. These results suggest that the intracellular distribution of αCP2 mRNA is developmentally regulated in a gene and/or cell specific manner.