Production of antioomycete compounds active against the phytopathogens Phytophthora sojae and Aphanomyces cochlioides by clavicipitoid entomopathogenic fungi

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• 作者：Sastia Prama Putri¹ ; Kei-ichi Ishido¹ ; Hiroshi Kinoshita¹ ; Shigeru Kitani¹ ; Fumio Ihara² ; Yasuko Sakihama⁴ ; Yasuhiro Igarashi³ ; Takuya Nihira¹ ; ⁵ ; ^{nihira@icb.osaka-u.ac.jp" class="auth_mail}
• 关键词：Antioomycete compounds ; Phytophthora sojae ; Aphanomyces cochlioides ; Clavicipitoid entomopathogenic fungi ; Bioactive compound
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：May, 2014
• 年：2014
• 卷：117
• 期：5
• 页码：557-562
• 全文大小：604 K

文摘

A total of 412 strains belonging to 14 genera of clavicipitoid entomopathogenic fungi (EPF) were screened for activities against two economically important plant pathogenic oomycetes, Phytophthora sojae and Aphanomyces cochlioides. To identify the antioomycete compounds produced by EPF, the extracts of 13 highly active EPF strains were characterized in detail by high performance liquid chromatography with diode array detection and high-resolution mass spectrometric detection and antioomycete assay. The antioomycete activity of several Metarhizium extracts was associated with previously isolated aurovertins, fungerin, N-(methyl-3-oxodec-6-enoyl)-2-pyrroline, and N-(methyl-3-oxodecanoyl)-2-pyrroline. The depsipeptide beauvericin was confirmed to be one of the active principles of three strains of Isaria tenuipes, which strongly inhibited mycelial growth of both P.聽sojae and A.聽cochlioides. Two known bioactive metabolites, paecilosetin and aranorosinol A, together with a novel and potent antioomycete compound, farinomalein, were isolated from the extracts of Isaria farinosa and all compounds were confirmed to have antioomycete activity. Identification of 8 antioomycete compounds from 13 clavicipitioid EPF demonstrated a new potential use of EPF as a source of compounds for the control of soil-borne plant pathogenic oomycetes.