Catechol derivatives inhibit the fibril formation of amyloid-¦Â peptides

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• 作者：Vu Thi  ; Huong¹ ; Toshinori  ; Shimanouchi¹ ; Naoya  ; Shimauchi¹ ; Hisashi  ; Yagi² ; Hiroshi  ; Umakoshi¹ ; Yuji  ; Goto² ; Ryoichi  ; Kuboi¹ ;   ; ^{msb@cheng.es.osaka-u.ac.jp}
• 关键词：Liposome ; Fibril formation ; Inhibitor ; Catechol derivatives ; Amyloid beta ; Alzheimer's disease
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2010
• 出版时间：June 2010
• 年：2010
• 卷：109
• 期：6
• 页码：629-634
• 全文大小：610 K

文摘

The inhibition of fibril formation of amyloid ¦Â proteins (A¦Â) would be attractive therapeutic targets for the treatment of Alzheimer's disease (AD). Dopamine (DA) and other catechol derivatives were used as inhibitory factors for A¦Â fibril formation. The fibril formation of A¦Â was monitored by Thioflavin T fluorescence, a transmission electron microscopy (TEM) and a total internal reflection fluorescence microscopy (TIRFM). Catechol and its derivatives showed the dose-dependent inhibitory effects on the spontaneous A¦Â fibril formation. The inhibitory activity depended on the chemical structure of catechol derivatives both in the presence and absence of the liposome a model of biomembrane. Formation of catechol quinone-conjugated-A¦Â adduct by a Schiff-base is a key step for the inhibition effect of A¦Â fibril formation.