Development of fluorometric reactive oxygen species assay for photosafety evaluation
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- 作者：Yoshiki Seto ; Hiroto Ohtake ; Masashi Kato ; Satomi Onoue ; onoue@u-shizuoka-ken.ac.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：CV ; coefficient of variation ; DBB ; o-dibenzoylbenzene ; DMSO ; dimethyl sulfoxide ; DPBF ; 1 ; 3-diphenylisobenzofuran ; fROS assay ; fluorometric reactive oxygen species assay ; H2O2 ; hydrogen peroxide ; ICH ; the International Council on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use ; mROS assay ; micellar reactive oxygen species assay ; NaPB ; sodium phosphate buffer ; NBT ; nitroblue tetrazolium ; OECD ; the Organisation for Economic Co-operation and Development ; PAB
- 刊名：Toxicology in Vitro
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：34
- 期：Complete
- 页码：113-119
- 全文大小：647 K
文摘
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Reactive oxygen species (ROS) assay has operational complexity and a long run time.
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Fluorescent ROS (fROS) assay was developed for improving these drawbacks of the ROS assay.
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Although only a few false negative were observed, the fROS assay could simplify assay procedures with enhanced productivity.
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Upon further improvement on fluorescent probe, the fROS assay would provide for photosafety assessment of chemicals.