A novel, rapid and efficient method of cloning functional antigen-specific T-cell receptors from single human and mouse T-cells
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- 作者：Hiroshi Hamana ; Kiyomi Shitaoka ; Hiroyuki Kishi ; immkishi@med.u-toyama.ac.jp" class="auth_mail" title="E-mail the corresponding author ; Tatsuhiko Ozawa ; Atsushi Muraguchi
- 关键词：T-cell receptor ; Multiplex one-step RT-PCR ; Transcriptionally active PCR ; Luciferase reporter assay
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2016
- 出版时间：10 June 2016
- 年：2016
- 卷：474
- 期：4
- 页码：709-714
- 全文大小：1610 K
文摘
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Novel system for the paired cloning of antigen-specific TCRαβ is presented.
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The system enables us to clone and analyze antigen-specific TCRs within four days.
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EB virus-specific TCRs and melanoma-associated antigen-specific TCRs are cloned.