Cord blood allergen-specific IgE is associated with reduced IFN-γ production by cord blood cells: The Protection against Allergy—Study in Rural Environments (PASTURE) study

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• 作者：Petra Ina Pfefferle ; Serdar Sel ; Markus Johannes Ege ; Gisela Bü ; chele ; Nicole Blü ; mer ; Susanne Krauss-Etschmann ; Ileana Herzum ; Christoph E. Albers ; Roger P. Lauener ; Marjut Roponen ; Maija-Riitta Hirvonen ; Dominique A. Vuitton ; Josef Riedler ; Bert Brunekreef ; Jean-Charles Dalphin ; Charlotte
• 关键词：Prenatal ; allergen specific IgE ; cord blood ; prospective birth cohort study ; farming environment
• 刊名：Journal of Allergy and Clinical Immunology
• 出版年：2008
• 出版时间：October 2008
• 年：2008
• 卷：122
• 期：4
• 页码：711-716
• 全文大小：160 K

文摘

Background

It is currently discussed whether allergic sensitization may start in utero under the influence of the maternal immune system and environmental determinants.

Objective

To investigate the relationship between allergen-specific cord blood (CB) IgE levels, parental sensitization, CB cytokine production, and environmental influences.

Methods

As part of an ongoing multicenter birth cohort study, allergen-specific IgE antibodies against 20 common seasonal, perennial, and food allergens were measured in blood samples from 922 neonates, 922 mothers, and 835 fathers. Supernatants from stimulated CB cells were assessed for the production of IL-5, IFN-γ, IL-10, and TNF-α.

Results

Allergen-specific IgE antibodies were detectable in 23.9 % of newborns. Contamination with maternal serum was excluded by several means of analyses, including the absence of IgA antibodies. Clear correlation between maternal and fetal IgE was found only for hen's egg, cow's milk, and soybean allergen. Fetal IgE correlated negatively with the level of IFN-γ production, but not with IL-5 and IL-10.

Conclusion

Allergen-specific IgE antibodies most probably of fetal origin are detectable in CB and correlate with a lowered CB IFN-γ production.