- 作者：Hisashi Shiga ; Takayuki Kajiura ; Junko Shinozaki ; Sho Takagi ; Yoshitaka Kinouchi ; Seiichi Takahashi ; Kenichi Negoro ; Katsuya Endo ; Yoichi Kakuta ; Manabu Suzuki ; Tooru Shimosegawa
- 关键词：Crohn's disease ; Elemental diet ; Intestinal microbiota ; T-RFLP
- 刊名：Digestive and Liver Disease
- 出版年：2012
- 出版时间：September, 2012
- 年：2012
- 卷：44
- 期：9
- 页码：736-742
- 全文大小：690 K
Background
Intestinal microbiota contributes to the pathogenesis of Crohn's disease. Elemental diet and total parenteral nutrition are effective therapies for Crohn's disease; however, changes of microbiota as a result of both treatments have not been fully elucidated.Aim
To elucidate changes of faecal microbiota in Crohn's disease patients treated with elemental diet and total parenteral nutrition.
Methods
Stool samples were collected from 33 active Crohn's disease patients and 17 healthy subjects, and recollected after elemental diet (8 patients) and total parenteral nutrition (9 patients). Terminal restriction fragment length polymorphism analysis of bacterial 16srDNA was performed to evaluate the whole microbiota. Specific quantitative PCR was then used to determine populations of predominant bacterial groups.
Results
In Crohn's disease patients, the number of terminal restriction fragments, which reflects bacterial species, was significantly lower. Populations of total bacteria and Bifidobacterium were significantly lower and the ratio of Enterococcus was higher. The number of terminal restriction fragments was significantly decreased after total parenteral nutrition, but not after elemental diet. Population of Bacteroides fragilis significantly decreased after elemental diet, while population of Enterococcus significantly increased after total parenteral nutrition.
Conclusion
Faecal microbiota in Crohn's disease patients was markedly different from healthy subjects. Species diversity was reduced by total parenteral nutrition, but not by elemental diet.