Simultaneous quantification of seven prostanoids using liquid chromatography/tandem mass spectrometry: The effects of arachidonic acid on prostanoid production in mouse bone marrow-derived mast cells

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• 作者：Takanori Hishinuma ; Kaori Suzuki ; Masayoshi Saito ; Hiroaki Yamaguchi ; Naoto Suzuki ; Yoshihisa Tomioka ; Izumi Kaneko ; Masao Ono ; Junichi Goto
• 关键词：Beraprost sodium ; Anti-inflammatory effect ; Pulmonary ; thromboembolism ; Lung edema and ventricular hypertrophy ; 5-HT-induced ; edema ; Prostaglandin E₁
• 刊名：Prostaglandins, Leukotrienes and Essential Fatty Acids
• 出版年：2007
• 出版时间：June 2007
• 年：2007
• 卷：76
• 期：6
• 页码：321-329
• 全文大小：330 K

文摘

We have developed a method for the simultaneous estimation of the levels of the prostanoids 6-keto prostaglandin (PG) F_lα, PGB₂, PGD₂, PGE₂, PGF_2α, PGJ₂, and thromboxane (TX) B₂ in blood- or serum-containing medium using liquid chromatography-tandem mass spectrometry. These prostanoids and their deuterium derivatives, which were used as internal standards, were subjected to solid-phase extraction using Empore C18 HD disk cartridges and analyzed in the selected reaction-monitoring mode. A linear response curve starting at 10 pg of prostanoid/tube was observed for each prostanoid. The accuracy of the method was demonstrated with samples containing known amounts of the prostanoids. Furthermore, we used this method to analyze the prostanoids produced in mouse bone marrow-derived mast cells stimulated with arachidonic acid, which resulted in the production of PGD₂, PGE₂, PGF_2α, and TXB₂. The results suggest that this simultaneous quantification method is useful for the analysis of the production of biomedically important prostanoids.