伪-Amylase from wheat (Triticum aestivum) seeds: Its purification, biochemical attributes and active site studies
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- 作者：Kritika Singh ; Arvind M. Kayastha ; kayasthabhu@gmail.com" class="auth_mail
- 关键词：伪-Amylase ; Glycoprotein ; Purification ; MS/MS ; SDS ; Chemical modification ; Histidine
- 刊名：Food Chemistry
- 出版年：1 November 2014
- 年：2014
- 卷：162
- 期：Complete
- 页码：1-9
- 全文大小：674 K
文摘
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Wheat 伪-amylase was purified to homogeneity and its identity confirmed by MALDI-TOF.
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Biochemical characterisation revealed resistance to SDS denaturation at low concentrations.
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High kcat objectifies wheat 伪-amylase for starch based and related industries.
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Chemical modification studies showed histidine presence at the enzyme’s active site.