Optimized assay for the quantification of histidine kinase autophosphorylation
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- 作者：Takahiro B. Uenoa ; Roger A. Johnsonb ; Elizabeth M. Boona ; elizabeth.boon@stonybrook.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Histidine kinase ; TCS ; Two-component signaling ; His-Asp phosphorelay ; Phospho-His ; Autophosphorylation ; Filter binding assay ; Autoradiography
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2015
- 出版时间：25 September 2015
- 年：2015
- 卷：465
- 期：3
- 页码：331-337
- 全文大小：812 K
文摘
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Two-component signaling (TCS) is essential to bacteria, but it is poorly characterized.
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Environment-responsive histidine phosphorylation is the initial step in TCS.
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We report a facile medium-throughput assay for quantifying histidine phosphorylation.
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Using this assay, we analyze the kinetics of many uncharacterized histidine kinases.