The FA composition of tissue obtained via different methods was compared to that of tissue collected via large-needle or surgical biopsy. Fatty acid composition was not significantly different in AT collected by small-needle mini-biopsy (<em>nem>聽=聽10), from an RNA 鈥榣ipid layer鈥?(obtained during RNA extraction, 2 sites, <em>nem>聽=聽6 for each), or from cryosectioned tissue prepared for histology (<em>nem>聽=聽10). We also assessed the usefulness of the composition of plasma NEFA as a surrogate marker of subcutaneous AT (<em>nem>聽=聽58-80). Most FAs in plasma NEFA correlated strongly with those in AT (<em>Pem>聽<聽0.05).
It is feasible to measure the FA composition of AT on very small amounts of tissue. Additionally, it is possible to measure FA composition on the lipid rich 鈥榖y-product鈥?of AT samples undergoing RNA extraction for gene expression. Samples sectioned for histology are also suitable. This provides further opportunities for multidisciplinary collaborations that may lead to a better application of dietary biomarkers.