Far-red fluorescence: A direct spectroscopic marker for LHCII oligomer formation in non-photochemical quenching
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- 作者：Yuliya Miloslavina ; Antje Wehner ; Petar H. Lambrev ; Emilie Wientjes ; Michael Reus ; Győ ; ző ; Garab ; Roberta Croce ; Alfred R. Holzwarth
- 关键词：LHCII ; Aggregation ; Chlorophyll fluorescence ; Non-photochemical quenching ; Violaxanthin ; Zeaxanthin ; Arabidopsis mutant
- 刊名：FEBS Letters
- 出版年：2008
- 出版时间：29 October 2008
- 年：2008
- 卷：582
- 期：25-26
- 页码：3625-3631
- 全文大小：224 K
文摘
Time-resolved fluorescence on oligomers of the main light-harvesting complex from higher plants indicate that in vitro oligomerization leads to the formation of a weakly coupled inter-trimer chlorophyll–chlorophyll (Chl) exciton state which converts in tens of ps into a state which is spectrally broad and has a strongly far-red enhanced fluorescence spectrum. Both its lifetime and spectrum show striking similarity with a 400 ps fluorescence component appearing in intact leaves of Arabidopsis when non-photochemical quenching (NPQ) is induced. The fluorescence components with high far-red/red ratio are thus a characteristic marker for NPQ conditions in vivo. The far-red emitting state is shown to be an emissive Chl–Chl charge transfer state which plays a crucial part in the quenching.