Efficient differentiation of murine embryonic stem cells requires the binding of CXXC finger protein 1 to DNA or methylated histone H3-Lys4
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- 作者：Jyothi Mahadevana ; David G. Skalnika ; b ; dskalnik@iupui.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：aa ; amino acid ; AP ; alkaline phosphatase ; Cfp1 ; CXXC finger protein 1 ; DAPI ; 4&prime ; 6-diamidino-2-phenylindone ; EB ; embryoid bodies ; ES ; embryonic stem ; GST ; glutathione S-transferase ; LIF ; leukemia inhibitory factor ; PHD ; plant homeodomain ; WT ; wild type
- 刊名：Gene
- 出版年：2016
- 出版时间：5 December 2016
- 年：2016
- 卷：594
- 期：1
- 页码：1-9
- 全文大小：1314 K
文摘
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The plant homeodomain (PHD) of Cfp1 directly binds to histone H3K4me1/me2/me3.
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Mutation of conserved residues within Cfp1 PHD ablates this binding activity.
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Cfp1-H3K4me binding is not required for rescue of Cfp1−/− ESC differentiation.
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ESC differentiation needs retention of either DNA or histone binding activity of Cfp1.