Simultaneous determination of plasma total homocysteine and methionine by liquid chromatography-tandem mass spectrometry

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• 作者：Yi Jiang^a ; Brandon Mistretta^a ; Sarah Elsea^a ; ^b ; Qin Sun^a ; ^b ; ^qsun@bcm.edu
• 关键词：Homocysteine ; Methionine ; Tandem mass spectrometry ; Homocystinuria ; cblC ; Sulfocysteinuria
• 刊名：Clinica Chimica Acta
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：464
• 期：Complete
• 页码：93-97
• 全文大小：542 K
• 卷排序：464

文摘

A simple LC-MS/MS method was developed and validated for determination of homocysteine and methionine in human plasma. Simultaneous measurement of methionine helps in the differentiation of homocystinuria and some cobalamin disorders (such as cblC and cblD defects) without additional amino acid testing. This assay is sensitive to detect reduced total homocysteine concentrations in sulfocysteinuria and molybdenum cofactor deficiencies.