DNA damage, repair monitoring and epigenetic DNA methylation changes in seedlings of Chernobyl soybeans

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• 作者：Mariyana Georgieva^a ; ^b ; ^{mimy80@abv.bg} ; Namik M. Rashydov^c ; Martin Hajduch^a
• 关键词：CNPP ; Chernobyl nuclear power plant ; IR ; ionizing radiation ; ROS ; reactive oxygen species ; SSBs ; single strand breaks ; DSBs ; double strand breaks ; R-C ; radio-contaminated ; DMSO ; dimethyl sulfoxide ; N/N ; neutral unwinding/neutral electrophoresis ; A/N ; alkaline unwinding/neutral electrophoresis ; A/A ; alkaline unwinding/alkaline electrophoresis ; SE ; standard errors ; SD ; standard deviation ; SNK ; Student-Newman-Keuls test ; ALS ; alkali-labile sites ; NHEJ ; non-homologous end joining
• 刊名：DNA Repair
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：50
• 期：Complete
• 页码：14-21
• 全文大小：1535 K
• 卷排序：50

文摘

External chronic radiation and internally incorporated radionuclides after Chernobyl accident induce genome instability in the plant genome. Induced DNA strand breaks were efficiently repaired at the soybean cotyledon stage. Development of a new experimental design of the Methylation-sensitive comet assay adapted for a plant model system. A slight fluctuation in the CCGG methylation pattern was connected with adaptation capacity in Chernobyl plants.