Determination of albumins by its quenching effect on the fluorescence of Tb³⁺-oxolinic acid complex in presence of sodium dodecyl sulphate

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• 作者：Xia Wu ; Jinhua Zheng ; ChangYing Guo ; Jinghe Yang ; Honghong Ding ; Zhiyong Hu ; Chao Li
• 关键词：Fluorescence quenching ; Albumin ; Tb³⁺-OA– ; SLS
• 刊名：Journal of Luminescence
• 出版年：2007
• 出版时间：September 2007
• 年：2007
• 卷：126
• 期：1
• 页码：171-176
• 全文大小：302 K

文摘

It is found that the fluorescence intensity of Tb³⁺-oxolinic acid (OA) complex can be greatly quenched by albumins in sodium dodecyl sulphate (SLS). Under optimum conditions, the quenched fluorescence intensity is in proportion to the concentration of proteins in the range of 5.0×10⁻⁸–1.0×10⁻⁵ g ml⁻¹ for bovine serum albumin (BSA), 1.0×10⁻⁷–1.0×10⁻⁵ g ml⁻¹ for human serum albumin (HSA) and 4.0×10⁻⁷–1.0×10⁻⁵ g ml⁻¹ for egg albumin (EA). Their detection limits (S/N=3) are 2.1×10⁻⁸, 2.5×10⁻⁸ and 5.0×10⁻⁸ g ml⁻¹, respectively. In addition, the interaction mechanism is also investigated.