Validation of a gas chromatography-electron capture detection of T-2 and HT-2 toxins in Chinese herbal medicines and related products after immunoaffinity column clean-up and pre-column derivatization

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• 作者：Weijun Kong^a ; ¹ ; Xiaofei Zhang^a ; ^b ; ¹ ; Honghong Shen^a ; ^b ; Zhen Ou-Yang^b ; Meihua Yang^a ; ^{yangmeihua15@hotmail.com}
• 关键词：GC-ECD ; GC&ndash ; MS ; Method validation ; T-2 and HT-2 toxins ; Chinese herbal medicine
• 刊名：Food Chemistry
• 出版年：2012
• 出版时间：1 May 2012
• 年：2012
• 卷：132
• 期：1
• 页码：574-581
• 全文大小：755 K

文摘

A sensitive, reproducible and accurate gas chromatography-electron capture detection (GC-ECD) method was developed for simultaneous determination of T-2 and HT-2 toxins in Chinese herbal medicines (CHMs) and related products after immunoaffinity column (IAC) clean-up and pre-column derivatization with N-heptafluoro-butyryl imidazole (HFBI). Then, gas chromatography-spectrometry spectrometer (GC-MS) was applied to confirm the positive results and interfering peaks. The limits of detection (LODs) for T-2 and HT-2 toxins were 1.88 and 0.47 ng/g, and the recoveries for different CHMs ranged from 89.2 % to 99.1 % with relative standard deviation (RSD) <6.0 % for T-2 and from 85.9 % to 99.0 % with RSD <8.8 % for HT-2 toxin, respectively. The validated method was successfully applied for the determination of T-2 and HT-2 toxins in 89 Chinese herbal medicines and 10 related products from various sources, where it was found that T-2 and HT-2 toxins were not detected in any of the tested samples. These results were reliable by confirmation using GC-MS. Some unknown peaks were interfering peaks not the target toxins.