A novel sandwich electrochemiluminescence immunosensor for ultrasensitive detection of carbohydrate antigen 19-9 based on immobilizing luminol on Ag@BSA core/shell microspheres
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- 作者：Amin Zhang ; Hongkun Xiang ; Xin Zhang ; Weiwei Guo ; Enhui Yuan ; Chusen Huang ; Nengqin Jia ; nqjia@shnu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Electrochemiluminescence ; Immunosensor ; Carbohydrate antigen 19-9 ; Luminol ; Ag@BSA core/shell microspheres ; Clinical assay
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 January 2016
- 年：2016
- 卷：75
- 期：Complete
- 页码：206-212
- 全文大小：2317 K
文摘
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An applicable and sensitive ECL immunosensor for CA19-9 was developed.
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Ag@BSA microspheres were used for effective cross-linking of Ab2 and luminol molecules.
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PEI-modified MAGCNTs was used to construct the base of the immunosensor and load plenty of Ab1.
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The ECL immunosensor displays a wide linear response and low detection limit toward CA19-9.
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The proposed ECL immunosensor was applied to detect CA19-9 in real samples.