Identification of splice variants, expression analysis and single nucleotide polymorphisms of the PRMT2 gene in dairy cattle

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• 作者：Zhixiong Li^a ; Mengxing Zhai^a ; Hongliang Wang^a ; Ling Chen^a ; Lijun Wang^a ; Caixia Ru^b ; Ailong Song^b ; Xiaolin Liu^a ; ^{xiaolinliu2000@sina.com" class="auth_mail}
• 关键词：AS ; alternative splicing ; aa ; amino acids ; Ne ; effective allele numbers ; He ; heterozygosity ; Ho ; homozygosity ; PCR-RFLP ; PCR-Restriction Fragment Length Polymorphism ; PCR-SSCP ; PCR-single strand conformation polymorphism ; PIC ; polymorphism information content ; PRMT2 ; protein arginine N-methyltransferase 2 ; Q-PCR ; quantitative-PCR ; RT-PCR ; reverse transcription-polymerase chain reaction ; SNP ; single nucleotide polymorphism ; SCC ; somatic cell count ; SCS ; somatic cell score ; SV ; splice variant
• 刊名：Gene
• 出版年：10 April, 2014
• 年：2014
• 卷：539
• 期：1
• 页码：37-43
• 全文大小：831 K

文摘

Protein arginine N-methyltransferase 2 (PRMT2), also named HRMT1L1, belongs to the Bovine Protein arginine N-methyltransferase (PRMT) genes which are involved in the immune response. To explore the variability of the PRMT2 gene and resistance to mastitis in cows, splice variant (SV), and single nucleotide polymorphisms (SNPs) were identified in this study. A SV (PRMT2-SV) lacking exon 7 (98-bp) of the PRMT2 gene was found in healthy and mastitis-infected mammary gland tissues. Two of four SNPs were significantly associated with bovine milk yield and protein content. Further, we estimated the relative expression of PRMT2-SV in the mammary gland tissue of dairy cattle by using quantitative real-time polymerase chain reaction. The result showed that expression of the PRMT2-SV mRNA was significantly upregulated 4.02-fold (p < 0.05) in infected mammary tissues (n = 5) compared to healthy tissues (n = 5). Our findings reveal that PRMT2-SV may play an important role in mastitis resistance in dairy cattle. The SNPs may be used as a possible candidate SNPs for marker-assisted selection and management in Chinese Holstein cattle.