IL-35 gene modified MSCs were constructed by lentivirus transfection and can produce large amounts of IL-35 in vitro. IL-35-MSCs can inhibit the proliferation of CD4+ T cells and IL-17A secretion. IL-35-MSCs also can induce IL-10 production by CD4+ T cells, but did not affect IFN-γ. IL-35-MSCs can increase the proportion of CD4+Foxp3+Treg cells.