Tissue turnover in ovine muscles and lipids as recorded by multiple (H, C, O, S) stable isotope ratios

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• 作者：Sabine M. Harrison ; Olaf Schmidt ; Aidan P. Moloney ; Simon D. Kelly ; Andreas Rossmann ; Antje Schellenberg ; Federica Camin ; Matteo Perini ; Jurian Hoogewerff ; Frank J. Monahan
• 关键词：Stable isotopes ; Carbon ; Sulphur ; Hydrogen ; Oxygen ; Meat ; Muscle ; Lipid ; Lamb ; IRMS ; Isotopic clock
• 刊名：Food Chemistry
• 出版年：2011
• 出版时间：1 January 2011
• 年：2011
• 卷：124
• 期：1
• 页码：291-297
• 全文大小：277 K

文摘

Multiple stable isotope ratios (δ²H, δ¹³C, δ¹⁸O and δ³⁴S) were measured in muscle, muscle lipids and lipid fractions collected from 28 lambs, subjected to a diet-switch and raised on two energy allowances (EAs), to determine tissue turnover and diet-tissue fractionation. The diet-muscle fractionations prior to the diet-switch were estimated to be −44.0‰, +1.9‰ and 0‰ for H, C and S, respectively, while the drinking water was demonstrated to be the main source of muscle O and thus δ¹⁸O variation. The diet-intra-muscular lipid fractionations prior to the diet-switch were estimated to be −172.7‰, −1.3‰ and −11.5‰ for H, C and O, respectively. The C half-lives of muscle were determined to be 75.7 and 91.6 days for animals receiving the high and low EA, respectively. Extracting temporally resolved pre-slaughter dietary information from meat by analysing bulk muscle, muscle lipids and muscle lipid fractions appeared to be not practicable due to possible incomplete turnover of lipids.