On the phosphorylase activity of GH3 enzymes: A β-N-acetylglucosaminidase from Herbaspirillum seropedicae SmR1 and a glucosidase from Saccharopolyspora erythraea
详细信息 查看全文
- 作者：Diogo R.B. Ducattia ; b ; Madison A. Carrollc ; David L. Jakemana ; c ; david.jakeman@dal.ca" class="auth_mail" title="E-mail the corresponding author
- 关键词：Glycoside hydrolase ; Glycosidase ; GH3 N-acetylglucosaminidases ; Phospho-sugar ; Herbaspirillum seropedicae SmR1 ; Chemical compounds
- 刊名：Carbohydrate Research
- 出版年：2016
- 出版时间：29 November 2016
- 年：2016
- 卷：435
- 期：Complete
- 页码：106-112
- 全文大小：752 K
文摘
- •
GH3 Hexosaminidase Hsero1941 from Herbaspirillum seropedicae SmR1 was characterized.
- •
Hsero1941 hydrolyzes ß-D-aryl glucosides and ß-D-glucosyl phosphates.
- •
GH3 Glucosidase EryBI showed hydrolytic activity against ß-D-glucosyl phosphate.
- •
Phosphorolytic activity is not observed by all beta-N-acetylglucosaminidases from GH3 family.
- •
A Histidine general acid/base is not indicative of phosphorolytic activity.