Expression and characterization of a fibrinogenolytic enzyme from horsefly salivary gland
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- 作者：Xun Shenga ; 1 ; Li Gaob ; 1 ; Xiancui Lub ; c ; 1 ; Yunfei Wangb ; c ; Yalan Hanb ; c ; Ping Mengb ; d ; e ; Wenlin Chenf ; chenwenlin@aliyun.com" class="auth_mail" title="E-mail the corresponding author ; Qiumin Lub ; d ; e ; lvqm@mail.kiz.ac.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Horsefly ; Salivary gland ; Fibrinogenolytic enzyme ; Anticoagulant ; SCP domain
- 刊名：Protein Expression and Purification
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：129
- 期：Complete
- 页码：135-142
- 全文大小：1768 K
文摘
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Tablysin 2 is a fibrinogenolytic enzyme from salivary glands of horsefly.
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Tablysin 2 was expressed in E. coli with pET-32a (+) plasmid as fusion protein.
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Tablysin 2 is anticoagulant by hydrolyzing fibrinogen and inhibiting platelets.
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A method for expression of trace proteins in horsefly salivary gland is presented.