文摘
The use of modified acrylic microspheres for matrix immobilization of HRP enzyme and vinyl ferrocene as a mediator had been successfully developed. This study revealed that an amperometric capsaicin biosensor fabricated from these materials provided a good linear range, storage stability, reproducibility, and low detection limit. This biosensor was successfully applied for evaluation of chilli hotness in chilli fruit sample, as well as in comparison with a standard method that employed the HPLC method.