Subcellular location of horseradish peroxidase in horseradish leaves treated with La(III), Ce(III) and Tb(III)

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• 作者：Yaxin Ye ; Lihong Wang ; Xiaohua Huang ; Tianhong Lu ; Xiaolan Ding ; Qing Zhou ; Shaofen Guo
• 关键词：Lanthanum ; Cerium ; Terbium ; Horseradish peroxidise ; Subcellular distribution ; Mesophyll cell
• 刊名：Ecotoxicology and Environmental Safety
• 出版年：2008
• 出版时间：November 2008
• 年：2008
• 卷：71
• 期：3
• 页码：677-684
• 全文大小：1660 K

文摘

The agricultural application of rare-earth elements (REEs) would promote REEs inevitably to enter in the environment and then to threaten the environmental safety and human health. Therefore, the distribution of the REEs ion, ¹⁴¹Ce(III) and effects of La(III), Ce(III) and Tb(III) on the distribution of horseradish peroxidase (HRP) in horseradish mesophyll cells were investigated with electron microscopic radioautography and transmission electron microscopic cytochemistry. It was found for the first time that REEs ions can enter into the mesophyll cells, deposit in both extra and intra-cellular. Compared to the normal condition, after the horseradish leaves treated with La(III) or Tb(III), HRP located on the tonoplast is decreased and HRP is mainly located on the cell wall, while HRP is mainly located on the plasma membrane after the horseradish leaves were treated with Ce(III). This also indicated that REEs ions may regulate the plant growth through changing the distribution of enzymes.