Different kinetics of circulating antibody-secreting cell responses after primary and booster oral immunizations: A tool for assessing immunological memory

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• 作者：Susannah Leach ; ^{susannah.leach@microbio.gu.se} ; Anna Lundgren ^{anna.lundgren@microbio.gu.se} ; Ann-Mari Svennerholm ^{ann-mari.svennerholm@microbio.gu.se}
• 关键词：ALS ; antibodies in lymphocyte supernatants ; ASC ; antibody-secreting cell ; CF ; colonization factor ; rCTB ; recombinant cholera toxin binding subunit ; ETEC ; enterotoxigenic Escherichia coli ; GM ; geometric mean ; HRP ; horseradish peroxidise ; PBMCs ; peripheral
• 刊名：Vaccine
• 出版年：2013
• 出版时间：26 June, 2013
• 年：2013
• 卷：31
• 期：30
• 页码：3035-3038
• 全文大小：442 K

文摘

We show that the kinetics of circulating IgA as well as IgG antibody-secreting cell (ASC) responses differs considerably after primary and booster vaccination with the oral cholera vaccine Dukoral^?, as determined by the antibody in lymphocyte supernatant (ALS) as well as ELISPOT methods. Thus, whereas the antitoxin ASC responses did not peak until 7-9 days after primary vaccination, peak responses to a second dose given after two weeks, or a single booster dose given 6 months to 14 years later, were recorded already after 4-5 days and then rapidly declined. Our results indicate that many previous studies reporting ASC results 7-10 days after repeated immunization may have substantially underestimated the magnitudes of the responses. The results also suggest that detection of peak ASC responses at an early time point after booster immunization can be used as a simple tool to assess immunological memory.