Guanine nucleotide induced conformational change of Cdc42 revealed by hydrogen/deuterium exchange mass spectrometry
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- 作者：Sheng-Wei Yanga ; Hsiu-Chi Tinga ; Yi-Ting Loa ; Ting-Yuan Wua ; Hung-Wei Huanga ; Chia-Jung Yangb ; Jui-Fen Riva Chana ; Min-Chieh Chuanga ; Yuan-Hao Howard Hsua ; c ; howardhsu@thu.edu.tw" class="auth_mail" title="E-mail the corresponding author
- 关键词：HDXMS ; amide hydrogen/deuterium exchange coupled with mass spectrometry ; P-loop ; phosphate binding loop ; GMP-PCP ; guanylyl β ; γ-methylene-diphosphate ; ODT ; 1-octadecanethiol
- 刊名：Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：1864
- 期：1
- 页码：42-51
- 全文大小：2360 K
文摘
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HDXMS revealed the dynamic changes of apo-Cdc42 upon guanine nucleotide binding.
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Guanine nucleotide binding decreased deuteration in P-loop, Switch II and α7 helix.
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GTP/GDP binding blocked the solvent accessibility in the active site.
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GTP/GDP binding induced a conformational change at the remote α7 helix.