Mastering analytical challenges for the characterization of pentacyclic triterpene mono- and diesters of Calendula officinalis flowers by non-aqueous C30 HPLC and hyphenation with APCI-QTOF-MS
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- 作者：Christoph Nicolausa ; 1 ; Adrian Sievers-Englerb ; 1 ; Renato Murilloc ; Michele D'Ambrosiod ; Michael Lä ; mmerhoferb ; michael.laemmerhofe@uni-tuebingen.de" class="auth_mail" title="E-mail the corresponding author ; Irmgard Merforta ; irmgard.merfort@pharmazie.uni-freiburg.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：Calendula officinalis ; Triterpene mono- and novel diesters ; (U)HPLC-APCI-QTOF-MS ; non-aqueous RPLC ; C30 stationary phase
- 刊名：Journal of Pharmaceutical and Biomedical Analysis
- 出版年：2016
- 出版时间：25 January 2016
- 年：2016
- 卷：118
- 期：Complete
- 页码：195-205
- 全文大小：1165 K
文摘
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Novel pentacyclic triterpene diesters isolated from Calendula officinalis flowers.
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Instability of triterpene mono- and diesters in ion-source makes their MS detection challenging.
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Problem solved by use of APCI and QTOF with specific instrumental settings.
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MS/MS scan with multi-channel averaging for pseudo-molecular ion detection of diesters.
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Non-aqueous C30 RP-HPLC for improved separation of diesters.