Replacement of two amino acids of 9R-dioxygenase-allene oxide synthase of Aspergillus niger inverts the chirality of the hydroperoxide and the allene oxide
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- 作者：Linda Soomana ; Anneli Wennmana ; Mats Hambergb ; Inga Hoffmanna ; Ernst H. Oliwa ; Ernst.Oliw@farmbio.uu.se" class="auth_mail" title="E-mail the corresponding author
- 关键词：AOS ; allene oxide synthase(s) ; CP ; chiral phase ; COX ; cyclooxygenase(s) ; CYP ; cytochrome P450 ; DiHODE ; dihydroxyoctadecadienoic acid ; DOX ; dioxygenase(s) ; EAS ; epoxyalcohol synthase ; 9(10)-EODE ; 9(10)-epoxy-10 ; 12(Z)-octadecadienoic acid ; 9 ; 10-DiHOME ; 9 ; 10-dihydroxy-12(Z)-octadecamonoenoic acid ; 9 ; 14-DiHODE ; 9 ; 14-dihydroxy-10(E) ; 12(E)-octadecadienoic acid ; 9-HPODE ; 9-hydroperoxy-10(E) ; 12(Z)-octadecadienoic acid ; HPOME ; hydroperoxyoctadecamonoenoic acid ; HPOTrE ; hydroperoxyoctadecatrienoic acid ; L
- 刊名：Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：1861
- 期：2
- 页码：108-118
- 全文大小：1669 K
文摘
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A set of dioxygenase-P450 fusion enzymes of Aspergilli remains to be characterized.
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Expression of one of these enzymes revealed a 9R-dioxygenase-allene oxide synthase.
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Sequence analysis showed homology to fungal 9S-dioxygenase-allene oxide synthases.
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Replacements in the 9R-dioxygenase domain could explain the catalytic differences.
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We conclude that 9R- and 9S- dioxygenase-allene oxide synthases are closely related.