Development and validation of molecular methodologies to assess PALB2 expression in sporadic breast cancer
详细信息 查看全文
- 作者：Nikoleta Poumpouridoua ; Amelia Acha-Sagredob ; Nikolaos Goutasc ; Dimitrios Vlachodimitropoulosc ; Ioanna Chatziioannidoua ; Evi Lianidoud ; Triantafyllos Lilogloub ; Christos Kroupisd ; ckroupis@med.uoa.gr" class="auth_mail" title="E-mail the corresponding author
- 关键词：CISH ; Chromogenic in situ hybridization ; Cq ; Quantification cycle ; CV ; Coefficient of variation ; FFPE ; formalin-fixed paraffin embedded tissue in blocks ; HR ; homologous recombination ; IHC ; Immunohistochemistry ; LOD ; limit of detection ; MLPA ; Multiplex Ligation-dependent Probe Amplification ; (MS-) HRMA ; (Methylation Sensitive-) High Resolution Melting curve Analysis ; PARP ; Poly (ADP Ribose) Polymerase ; PCR ; Polymerase Chain Reaction ; PTT ; Protein Truncation Test ; qMSP ; quantitative Methylation sp
- 刊名：Clinical Biochemistry
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：49
- 期：3
- 页码：253-259
- 全文大小：622 K
文摘
- •
PALB2 gene is included in the growing list of hereditary breast cancer genes.
- •
Novel molecular assays were developed in order to assess PALB2 expression in sporadic breast cancer.
- •
PALB2 promoter was not methylated in any of the 91 samples tested with pyrosequencing.
- •
95.6% of breast tumors were positive for PALB2 mRNA expression in a RT-qPCR assay.
- •
No significant correlation was found between PALB2 mRNA levels and histopathology.