Visibility of lipid resonances in HR-MAS spectra of brain biopsies subject to spinning rate variation
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- 作者：C. Prechta ; christina.precht@vetsuisse.unibe.ch" class="auth_mail" title="E-mail the corresponding author ; G. Diserensb ; A. Oevermannc ; M. Vermathend ; J. Langa ; C. Boeschb ; P. Vermathenb
- 关键词：NMR spectroscopy ; Gray matter ; White matter ; Lipid ; Nile Red
- 刊名：Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：1851
- 期：12
- 页码：1539-1544
- 全文大小：978 K
文摘
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We investigate lipids in healthy sheep brain biopsies by HRMAS NMR and histopathology.
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Lipid signal intensities increase as a function of spinning rate.
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Higher lipid peaks are observed in white compared to gray matter.
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Lipid staining suggests that lipid resonances originate from myelin plasma membranes.
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The results suggest the potential of the method for investigating lipids in myelin.